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人抗狂犬病毒抗體酶免試劑盒,(anti-RV)ELISA檢測試劑盒 ,英文名:anti-RV ELISA Kit ,英文縮寫:anti-RV ,常見試劑盒規(guī)格:96T/48T,產(chǎn)品別名:人抗狂犬病毒抗體ELISA檢測試劑盒,anti-RV 檢測試劑盒, anti-RV 檢測試劑盒
ELISA實驗操作較繁雜,操作不當(dāng)將引起較大的誤差。人抗狂犬病毒抗體酶免試劑盒,(anti-RV)ELISA檢測試劑盒在操作中應(yīng)注意以下5個方面。
1.隨著病情的進展或由其他因素影響,某些抗體在機體內(nèi)的含量發(fā)生大幅波動,因此有必要對標(biāo)本進行預(yù)處理,例如對血清標(biāo)本作適當(dāng)稀釋,或離心分離血脂等。間接法測定中,標(biāo)本適當(dāng)稀釋還可降低非特異性反應(yīng)。
2.加樣一般使用加液器,在ELISA中一般有4次加樣:加標(biāo)本、加酶結(jié)合物、加底物和加終止液。加標(biāo)本時必須每份標(biāo)本使用一支移液器吸嘴,以免交叉污染。加酶結(jié)合物、底物和終止液時則不需更換吸嘴。
3.在成批手工檢測中,還應(yīng)注意操作時差的影響。加樣及混勻時間的差異,使抗原抗體反應(yīng)和酶促反應(yīng)時間不*,對競爭法及定量檢測影響很大,不注意可能會導(dǎo)致錯誤結(jié)果或定量不準(zhǔn)。
4.洗滌是ELISA操作過程中決定實驗成敗的一個關(guān)鍵步驟。目的是除去未結(jié)合的免疫反應(yīng)物,終止抗原抗體反應(yīng),除去標(biāo)本中與反應(yīng)無關(guān)的成分、游離的酶標(biāo)物以及反應(yīng)過程中吸附于固相載體上的非特異性物質(zhì)。可用洗板機洗板或手工洗板,前者洗滌質(zhì)量較好,各反應(yīng)孔洗滌效果均一,批內(nèi)、批間差異小,手工洗板應(yīng)注意控制各孔洗滌效果,差異不宜太大。
5.準(zhǔn)確判定結(jié)果須使用ELISA測讀儀(酶標(biāo)儀),比色法判讀可選擇單波長或雙波長,根據(jù)反應(yīng)液顏色的不同選用不同波長的濾光片,以空白孔校零測定反應(yīng)孔的吸光度值(A值)。酶標(biāo)儀具有良好的重
抗脫氧核糖核酸酶B(Anti-DNaseB)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Anti-Deoxyribonuclease B Antibody (Anti-DNaseB)48T/96TY09335Homo sapiens (Human)
抗神經(jīng)元核抗體1型(ANNA1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Anti-Neuronal Nuclear Antibody 1 (ANNA1)48T/96TY09336Homo sapiens (Human)
X-射線修復(fù)交叉互補蛋白6(XRCC6)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for X-Ray Repair Cross Complementing 6 (XRCC6)48T/96TY09337Homo sapiens (Human)
染色質(zhì)域解旋酶DNA結(jié)合蛋白3(CHD3)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Chromodomain Helicase DNA Binding Protein 3 (CHD3)48T/96TY09338Homo sapiens (Human)
顆粒酶M(GZMM)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Granzyme M (GZMM)48T/96TY09339Homo sapiens (Human)
抗核膜糖蛋白210抗體(AGPA)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Anti-Glycoprotein 210 Antibody (AGPA)48T/96TY09340Homo sapiens (Human)
蛋白激酶Cδ(PKCδ)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Protein Kinase C Delta (PKCd)48T/96TY09341Homo sapiens (Human)
血小板衍生生長因子AB(PDGFAB)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Plaet Derived Growth Factor AB (PDGFAB)48T/96TY09342Homo sapiens (Human)
膽囊收縮素B受體(CCKBR)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Cholecystokinin B Receptor (CCKBR)48T/96TY09343Mus musculus (Mouse)
半胱氨酸蛋白酶抑制劑A(CSTA)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Cystatin A (CSTA)48T/96TY09344Homo sapiens (Human)
基質(zhì)細(xì)胞衍生因子2(SDF2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Stromal Cell Derived Factor 2 (SDF2)48T/96TY09345Homo sapiens (Human)
胸腺因子(TF)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Thymic Factor (TF)48T/96TY09346Homo sapiens (Human)
胎盤蛋白13(PP13)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Placental Protein 13 (PP13)48T/96TY09347Homo sapiens (Human)
核糖核酸抑制因子(RI)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Ribonuclease Inhibitor (RI)48T/96TY09348Homo sapiens (Human)
絲裂原激活蛋白激酶支架蛋白1(MAPKSP1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Mitogen Activated Protein Kinase Scaffold Protein 1 (MAPKSP1)48T/96TY09349Homo sapiens (Human)
早期生長應(yīng)答因子3(EGR3)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Early Growth Response Protein 3 (EGR3)48T/96TY09350Homo sapiens (Human)
細(xì)胞外信號調(diào)節(jié)激酶2(ERK2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Extracellular Signal Regulated Kinase 2 (ERK2)48T/96TY09351Homo sapiens (Human)
細(xì)胞外信號調(diào)節(jié)激酶2(ERK2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Extracellular Signal Regulated Kinase 2 (ERK2)48T/96TY09352Rattus norvegicus (Rat)
細(xì)胞外信號調(diào)節(jié)激酶2(ERK2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Extracellular Signal Regulated Kinase 2 (ERK2)48T/96TY09353Mus musculus (Mouse)
蛋白激酶Cα相互作用蛋白1(PICK1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Protein Interacting With Protein Kinase C Alpha 1 (PICK1)48T/96TY09354Homo sapiens (Human)
蛋白激酶Cα相互作用蛋白1(PICK1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Protein Interacting With Protein Kinase C Alpha 1 (PICK1)48T/96TY09355Rattus norvegicus (Rat)
蛋白激酶Cα相互作用蛋白1(PICK1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Protein Interacting With Protein Kinase C Alpha 1 (PICK1)48T/96TY09356Mus musculus (Mouse)
基質(zhì)金屬蛋白酶23B(MMP23B)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Matrix Metalloproteinase 23B (MMP23B)48T/96TY09357Homo sapiens (Human)
基質(zhì)金屬蛋白酶23B(MMP23B)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Matrix Metalloproteinase 23B (MMP23B)48T/96TY09358Rattus norvegicus (Rat)
間隙連接蛋白26(CX26)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Connexin 26 (CX26)48T/96TY09359Homo sapiens (Human)
堿性磷酸酶(ALP)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Alkaline Phosphatase (ALP)48T/96TY09360Homo sapiens (Human)
加溶金屬蛋白酶1(MP1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Pitrilysin Metalloproteinase 1 (MP1)48T/96TY09361Homo sapiens (Human)
基質(zhì)Gla蛋白(MGP)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Matrix Gla Protein (MGP)48T/96TY09362Homo sapiens (Human)
破骨細(xì)胞刺激因子1(OSF)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Osteoclast Stimulating Factor 1 (OSF)48T/96TY09363Homo sapiens (Human)
肝素結(jié)合性表皮生長因子(HBEGF)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Heparin Binding Epidermal Growth Factor Like Growth Factor (HBEGF)48T/96TY09364Homo sapiens (Human)
饑餓素(GHRL)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Ghrelin (GHRL)48T/96TY09365Homo sapiens (Human)
饑餓素(GHRL)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Ghrelin (GHRL)48T/96TY09366Rattus norvegicus (Rat)
饑餓素(GHRL)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Ghrelin (GHRL)48T/96TY09367Mus musculus (Mouse)
δ-促睡眠肽(δSIP)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Delta-Sleep Inducing Peptide (dSIP)48T/96TY09368Homo sapiens (Human)
醛固酮合酶(ALDOS)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Aldosterone Synthase (ALDOS)48T/96TY09369Rattus norvegicus (Rat)
Toll樣受體9(TLR9)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Toll Like Receptor 9 (TLR9)48T/96TY09370Homo sapiens (Human)
血管生成素2(ANGPT2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Angiopoietin 2 (ANGPT2)48T/96TY09371Oryctolagus cuniculus (Rabbit)
210kDa核孔蛋白(NUP210)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Nucleoporin 210kDa (NUP210)48T/96TY09372Rattus norvegicus (Rat)
210kDa核孔蛋白(NUP210)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Nucleoporin 210kDa (NUP210)48T/96TY09373Mus musculus (Mouse)
胰島素自身抗體(IAA)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Insulin Autoantibody (IAA)48T/96TY09374Mus musculus (Mouse)
胰島素自身抗體(IAA)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Insulin Autoantibody (IAA)48T/96TY09375Rattus norvegicus (Rat)
抑瘤素M受體(OSMR)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Oncostatin M Receptor (OSMR)48T/96TY09376Rattus norvegicus (Rat)
基質(zhì)金屬蛋白酶2(MMP2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Matrix Metalloproteinase 2 (MMP2)48T/96TY09377Oryctolagus cuniculus (Rabbit)
速激肽受體3(TACR3)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Tachykinin Receptor 3 (TACR3)48T/96TY09378Homo sapiens (Human)
白介素1受體拮抗劑(IL1RA)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Interleukin 1 Receptor Antagonist (IL1RA)48T/96TY09379Mus musculus (Mouse)
白介素1受體拮抗劑(IL1RA)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Interleukin 1 Receptor Antagonist (IL1RA)48T/96TY09380Homo sapiens (Human)
白介素1受體拮抗劑(IL1RA)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Interleukin 1 Receptor Antagonist (IL1RA)48T/96TY09381Rattus norvegicus (Rat)
趨化素(CHEM)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Chemerin (CHEM)48T/96TY09382Homo sapiens (Human)
Ⅰ型前膠原(PCⅠ)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Procollagen I (PCI)48T/96TY09383Rattus norvegicus (Rat)
ATP結(jié)合盒轉(zhuǎn)運蛋白G2(ABCG2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for ATP Binding Cassette Transporter G2 (ABCG2)48T/96TY09384Homo sapiens (Human)
各種類型ELISA測定時, 一般需經(jīng)過以下步驟:固相包被→加待測樣品→溫育→洗滌+加酶標(biāo)物→溫育→洗滌→加底物→溫育一加終止液→比色→判定結(jié)果。