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人大腸菌素酶免試劑盒,(colicin)ELISA檢測試劑盒,英文名:colicin ELISA Kit,英文縮寫:colicin ,常見試劑盒規(guī)格:96T/48T,產(chǎn)品別名:人大腸菌素ELISA檢測試劑盒,colicin 檢測試劑盒,colicin 檢測試劑盒
ELISA實驗操作較繁雜,操作不當(dāng)將引起較大的誤差。人大腸菌素酶免試劑盒,(colicin)ELISA檢測試劑盒在操作中應(yīng)注意以下5個方面。
1.隨著病情的進(jìn)展或由其他因素影響,某些抗體在機體內(nèi)的含量發(fā)生大幅波動,因此有必要對標(biāo)本進(jìn)行預(yù)處理,例如對血清標(biāo)本作適當(dāng)稀釋,或離心分離血脂等。間接法測定中,標(biāo)本適當(dāng)稀釋還可降低非特異性反應(yīng)。
2.加樣一般使用加液器,在ELISA中一般有4次加樣:加標(biāo)本、加酶結(jié)合物、加底物和加終止液。加標(biāo)本時必須每份標(biāo)本使用一支移液器吸嘴,以免交叉污染。加酶結(jié)合物、底物和終止液時則不需更換吸嘴。
3.在成批手工檢測中,還應(yīng)注意操作時差的影響。加樣及混勻時間的差異,使抗原抗體反應(yīng)和酶促反應(yīng)時間不*,對競爭法及定量檢測影響很大,不注意可能會導(dǎo)致錯誤結(jié)果或定量不準(zhǔn)。
4.洗滌是ELISA操作過程中決定實驗成敗的一個關(guān)鍵步驟。目的是除去未結(jié)合的免疫反應(yīng)物,終止抗原抗體反應(yīng),除去標(biāo)本中與反應(yīng)無關(guān)的成分、游離的酶標(biāo)物以及反應(yīng)過程中吸附于固相載體上的非特異性物質(zhì)。可用洗板機洗板或手工洗板,前者洗滌質(zhì)量較好,各反應(yīng)孔洗滌效果均一,批內(nèi)、批間差異小,手工洗板應(yīng)注意控制各孔洗滌效果,差異不宜太大。
5.準(zhǔn)確判定結(jié)果須使用ELISA測讀儀(酶標(biāo)儀),比色法判讀可選擇單波長或雙波長,根據(jù)反應(yīng)液顏色的不同選用不同波長的濾光片,以空白孔校零測定反應(yīng)孔的吸光度值(A值)。酶標(biāo)儀具有良好的重復(fù)性。
Apelin 13蛋白(AP13)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Apelin 13 (AP13)48T/96TY10135Homo sapiens (Human)
周期素依賴性激酶1(CDK1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Cyclin Dependent Kinase 1 (CDK1)48T/96TY10136Homo sapiens (Human)
周期素依賴性激酶2(CDK2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Cyclin Dependent Kinase 2 (CDK2)48T/96TY10137Homo sapiens (Human)
周期素依賴性激酶7(CDK7)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Cyclin Dependent Kinase 7 (CDK7)48T/96TY10138Homo sapiens (Human)
甘露糖結(jié)合蛋白(MBL)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Mannose Binding Lectin (MBL)48T/96TY10139Rattus norvegicus (Rat)
代謝型型谷氨酸受體3(GRM3)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Glutamate Receptor, Metabotropic 3 (GRM3)48T/96TY10140Homo sapiens (Human)
Salusinα蛋白(Salusinα)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Salusin Alpha48T/96TY10141Homo sapiens (Human)
血管內(nèi)皮生長因子受體3(VEGFR3)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Vascular Endothelial Growth Factor Receptor 3 (VEGFR3)48T/96TY10142Homo sapiens (Human)
Bcl2關(guān)聯(lián)X蛋白(Bax)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Bcl2 Associated X Protein (Bax)48T/96TY10143Rattus norvegicus (Rat)
干擾素γ(IFNγ)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Interferon Gamma (IFNg)48T/96TY10144Mus musculus (Mouse)
干擾素γ(IFNγ)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Interferon Gamma (IFNg)48T/96TY10145Rattus norvegicus (Rat)
胰島素樣生長因子1(IGF1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Insulin Like Growth Factor 1 (IGF1)48T/96TY10146Homo sapiens (Human)
胰島素樣生長因子1(IGF1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Insulin Like Growth Factor 1 (IGF1)48T/96TY10147Mus musculus (Mouse)
胰島素樣生長因子1(IGF1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Insulin Like Growth Factor 1 (IGF1)48T/96TY10148Rattus norvegicus (Rat)
胰島素樣生長因子2(IGF2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Insulin Like Growth Factor 2 (IGF2)48T/96TY10149Homo sapiens (Human)
胰島素樣生長因子2(IGF2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Insulin Like Growth Factor 2 (IGF2)48T/96TY10150Mus musculus (Mouse)
胰島素樣生長因子2(IGF2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Insulin Like Growth Factor 2 (IGF2)48T/96TY10151Rattus norvegicus (Rat)
胰島素樣生長因子結(jié)合蛋白1(IGFBP1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Insulin Like Growth Factor Binding Protein 1 (IGFBP1)48T/96TY10152Homo sapiens (Human)
胰島素樣生長因子結(jié)合蛋白1(IGFBP1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Insulin Like Growth Factor Binding Protein 1 (IGFBP1)48T/96TY10153Mus musculus (Mouse)
胰島素樣生長因子結(jié)合蛋白2(IGFBP2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Insulin Like Growth Factor Binding Protein 2 (IGFBP2)48T/96TY10154Homo sapiens (Human)
胰島素樣生長因子結(jié)合蛋白2(IGFBP2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Insulin Like Growth Factor Binding Protein 2 (IGFBP2)48T/96TY10155Mus musculus (Mouse)
胰島素樣生長因子結(jié)合蛋白3(IGFBP3)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Insulin Like Growth Factor Binding Protein 3 (IGFBP3)48T/96TY10156Homo sapiens (Human)
胰島素樣生長因子結(jié)合蛋白3(IGFBP3)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Insulin Like Growth Factor Binding Protein 3 (IGFBP3)48T/96TY10157Mus musculus (Mouse)
胰島素樣生長因子結(jié)合蛋白4(IGFBP4)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Insulin Like Growth Factor Binding Protein 4 (IGFBP4)48T/96TY10158Homo sapiens (Human)
白介素10(IL10)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Interleukin 10 (IL10)48T/96TY10159Homo sapiens (Human)
白介素10(IL10)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Interleukin 10 (IL10)48T/96TY10160Mus musculus (Mouse)
白介素10(IL10)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Interleukin 10 (IL10)48T/96TY10161Rattus norvegicus (Rat)
白介素11(IL11)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Interleukin 11 (IL11)48T/96TY10162Homo sapiens (Human)
白介素11(IL11)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Interleukin 11 (IL11)48T/96TY10163Mus musculus (Mouse)
白介素12B(IL12B)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Interleukin 12B (IL12B)48T/96TY10164Homo sapiens (Human)
白介素12A(IL12A)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Interleukin 12A (IL12A)48T/96TY10165Homo sapiens (Human)
白介素12A(IL12A)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Interleukin 12A (IL12A)48T/96TY10166Mus musculus (Mouse)
白介素13(IL13)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Interleukin 13 (IL13)48T/96TY10167Homo sapiens (Human)
白介素13(IL13)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Interleukin 13 (IL13)48T/96TY10168Mus musculus (Mouse)
白介素15(IL15)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Interleukin 15 (IL15)48T/96TY10169Homo sapiens (Human)
白介素15(IL15)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Interleukin 15 (IL15)48T/96TY10170Mus musculus (Mouse)
白介素16(IL16)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Interleukin 16 (IL16)48T/96TY10171Homo sapiens (Human)
白介素16(IL16)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Interleukin 16 (IL16)48T/96TY10172Mus musculus (Mouse)
白介素17(IL17)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Interleukin 17 (IL17)48T/96TY10173Chicken (Gallus)
白介素17(IL17)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Interleukin 17 (IL17)48T/96TY10174Mus musculus (Mouse)
白介素18(IL18)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Interleukin 18 (IL18)48T/96TY10175Mus musculus (Mouse)
白介素18(IL18)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Interleukin 18 (IL18)48T/96TY10176Rattus norvegicus (Rat)
白介素1受體Ⅱ(IL1R2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Interleukin 1 Receptor Type II (IL1R2)48T/96TY10177Homo sapiens (Human)
白介素1受體Ⅰ(IL1R1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Interleukin 1 Receptor Type I (IL1R1)48T/96TY10178Homo sapiens (Human)
白介素1α(IL1α)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Interleukin 1 Alpha (IL1a)48T/96TY10179Homo sapiens (Human)
白介素1α(IL1α)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Interleukin 1 Alpha (IL1a)48T/96TY10180Mus musculus (Mouse)
白介素1α(IL1α)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Interleukin 1 Alpha (IL1a)48T/96TY10181Rattus norvegicus (Rat)
層粘連蛋白β1(LAMβ1)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Laminin Beta 1 (LAMb1)48T/96TY10182Chicken (Gallus)
巨噬細(xì)胞炎性蛋白1α(MIP1α)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Macrophage Inflammatory Protein 1 Alpha (MIP1a)48T/96TY10183Rhesus monkey (Simian)
白介素2(IL2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Interleukin 2 (IL2)48T/96TY10184Homo sapiens (Human)
各種類型ELISA測定時,一般需經(jīng)過以下步驟:固相包被→加待測樣品→溫育→洗滌+加酶標(biāo)物→溫育→洗滌→加底物→溫育一加終止液→比色→判定結(jié)果。