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小鼠白介素2酶免試劑盒,(IL-2)ELISA檢測試劑盒,英文名:IL-2 ELISA Kit,英文縮寫:IL-2 ,常見試劑盒規格:96T/48T,產品別名:小鼠白介素2ELISA檢測試劑盒,IL-2 檢測試劑盒,IL-2 檢測試劑盒
ELISA實驗操作較繁雜,操作不當將引起較大的誤差。小鼠白介素2酶免試劑盒,(IL-2)ELISA檢測試劑盒在操作中應注意以下5個方面。
1.隨著病情的進展或由其他因素影響,某些抗體在機體內的含量發生大幅波動,因此有必要對標本進行預處理,例如對血清標本作適當稀釋,或離心分離血脂等。間接法測定中,標本適當稀釋還可降低非特異性反應。
2.加樣一般使用加液器,在ELISA中一般有4次加樣:加標本、加酶結合物、加底物和加終止液。加標本時必須每份標本使用一支移液器吸嘴,以免交叉污染。加酶結合物、底物和終止液時則不需更換吸嘴。
3.在成批手工檢測中,還應注意操作時差的影響。加樣及混勻時間的差異,使抗原抗體反應和酶促反應時間不*,對競爭法及定量檢測影響很大,不注意可能會導致錯誤結果或定量不準。
4.洗滌是ELISA操作過程中決定實驗成敗的一個關鍵步驟。目的是除去未結合的免疫反應物,終止抗原抗體反應,除去標本中與反應無關的成分、游離的酶標物以及反應過程中吸附于固相載體上的非特異性物質??捎孟窗鍣C洗板或手工洗板,前者洗滌質量較好,各反應孔洗滌效果均一,批內、批間差異小,手工洗板應注意控制各孔洗滌效果,差異不宜太大。
5.準確判定結果須使用ELISA測讀儀(酶標儀),比色法判讀可選擇單波長或雙波長,根據反應液顏色的不同選用不同波長的濾光片,以空白孔校零測定反應孔的吸光度值(A值)。酶標儀具有良好的重復性。
肌腱蛋白X(TNX)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Tenascin X (TNX)48T/96TY11085Rattus norvegicus (Rat)
肌腱蛋白X(TNX)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Tenascin X (TNX)48T/96TY11086Mus musculus (Mouse)
谷胱甘肽過氧化酶1(GPX1)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Glutathione Peroxidase 1 (GPX1)48T/96TY11087Mus musculus (Mouse)
黑色素瘤抗原家族A1(MAGEA1)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Melanoma Antigen Family A1 (MAGEA1)48T/96TY11088Mus musculus (Mouse)
芳香胺-N-乙?;D移酶(AANAT)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Arylalkylamine-N-Acetyltransferase (AANAT)48T/96TY11089Homo sapiens (Human)
肝腎骨堿性磷酸酶(ALPL)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Alkaline Phosphatase, Liver/Bone/Kidney (ALPL)48T/96TY11090Mus musculus (Mouse)
多配體蛋白聚糖4(SDC4)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Syndecan 4 (SDC4)48T/96TY11091Homo sapiens (Human)
組織因子通道抑制因子2(TFPI2)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Tissue Factor Pathway Inhibitor 2 (TFPI2)48T/96TY11092Homo sapiens (Human)
免疫球蛋白D(IgD)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Immunoglobulin D (IgD)48T/96TY11093Homo sapiens (Human)
肌酸激酶MB同工酶(CKMB)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Creatine Kinase MB Isoenzyme (CKMB)48T/96TY11094Mus musculus (Mouse)
肌酸激酶MB同工酶(CKMB)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Creatine Kinase MB Isoenzyme (CKMB)48T/96TY11095Rattus norvegicus (Rat)
α1-抗胰蛋白酶(α1AT)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Alpha-1-Antitrypsin (a1AT)48T/96TY11096Mus musculus (Mouse)
血小板衍生生長因子B(PDGFB)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Plaet Derived Growth Factor Subunit B (PDGFB)48T/96TY11097Sus scrofa; Porcine (Pig)
血管內皮生長因子A(VEGFA)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Vascular Endothelial Growth Factor A (VEGFA)48T/96TY11098Sus scrofa; Porcine (Pig)
叉頭框蛋白M1(FOXM1)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Forkhead Box Protein M1 (FOXM1)48T/96TY11099Mus musculus (Mouse)
神經調節素1(NRG1)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Neuregulin 1 (NRG1)48T/96TY11100Rattus norvegicus (Rat)
神經調節素1(NRG1)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Neuregulin 1 (NRG1)48T/96TY11101Mus musculus (Mouse)
膜聯蛋白A2(ANXA2)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Annexin A2 (ANXA2)48T/96TY11102Homo sapiens (Human)
表皮調節素(EREG)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Epiregulin (EREG)48T/96TY11103Homo sapiens (Human)
神經纖維素2(NF2)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Neurofibromin 2 (NF2)48T/96TY11104Mus musculus (Mouse)
心型脂肪酸結合蛋白(FABP3)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Fatty Acid Binding Protein 3, Muscle And Heart (FABP3)48T/96TY11105Rattus norvegicus (Rat)
頂體蛋白(ACR)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Acrosin (ACR)48T/96TY11106Homo sapiens (Human)
精子蛋白17(Sp17)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Sperm Protein 17 (Sp17)48T/96TY11107Homo sapiens (Human)
轉化生長因子β3(TGFβ3)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Transforming Growth Factor Beta 3 (TGFb3)48T/96TY11108Mus musculus (Mouse)
血管內皮生長因子受體2(VEGFR2)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Vascular Endothelial Growth Factor Receptor 2 (VEGFR2)48T/96TY11109Mus musculus (Mouse)
Toll樣受體7(TLR7)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Toll Like Receptor 7 (TLR7)48T/96TY11110Mus musculus (Mouse)
Toll樣受體7(TLR7)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Toll Like Receptor 7 (TLR7)48T/96TY11111Homo sapiens (Human)
胰腺再生蛋白Ⅳ(REG4)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Regenerating Islet Derived Protein 4 (REG4)48T/96TY11112Rattus norvegicus (Rat)
組蛋白乙?;D移酶1(HAT1)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Histone Acetyltransferase 1 (HAT1)48T/96TY11113Rattus norvegicus (Rat)
核因子κB激酶抑制因子相互作用蛋白(IκBIP)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Inhibitor Of Nuclear Factor Kappa B Kinase Interacting Protein (IKBIP)48T/96TY11114Homo sapiens (Human)
單氨氧化酶A(MAOA)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Monoamine Oxidase A (MAOA)48T/96TY11115Mus musculus (Mouse)
白介素17B(IL17B)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Interleukin 17B (IL17B)48T/96TY11116Homo sapiens (Human)
吲哚胺2,3-雙加氧酶(IDO)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Indoleamine-2,3-Dioxygenase (IDO)48T/96TY11117Rattus norvegicus (Rat)
丙酮酸脫氫酶α(PDHα)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Pyruvate Dehydrogenase Alpha (PDHa)48T/96TY11118Rattus norvegicus (Rat)
谷胱甘肽S轉移酶α2(GSTα2)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Glutathione S Transferase Alpha 2 (GSTa2)48T/96TY11119Homo sapiens (Human)
中期因子(MK)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Midkine (MK)48T/96TY11120Mus musculus (Mouse)
胃泌素(GT)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Gastrin (GT)48T/96TY11121Mus musculus (Mouse)
CD34分子(CD34)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Cluster Of Differentiation 34 (CD34)48T/96TY11122Homo sapiens (Human)
CD34分子(CD34)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Cluster Of Differentiation 34 (CD34)48T/96TY11123Mus musculus (Mouse)
促腎上腺皮質激素(ACTH)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Adrenocorticotropic Hormone (ACTH)48T/96TY11124Canis familiaris; Canine (Dog)
超氧化物歧化酶1(SOD1)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Superoxide Dismutase 1, Soluble (SOD1)48T/96TY11125Homo sapiens (Human)
心鈉肽(ANP)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Atrial Natriuretic Peptide (ANP)48T/96TY11126Rattus norvegicus (Rat)
心鈉肽(ANP)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Atrial Natriuretic Peptide (ANP)48T/96TY11127Canis familiaris; Canine (Dog)
心鈉肽(ANP)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Atrial Natriuretic Peptide (ANP)48T/96TY11128Oryctolagus cuniculus (Rabbit)
發動蛋白1(DNM1)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Dynamin 1 (DNM1)48T/96TY11129Homo sapiens (Human)
別孕烯醇酮(AP)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Allopregnanolone (AP)48T/96TY11130General
防御素β1(DEFβ1)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Defensin Beta 1 (DEFb1)48T/96TY11131Homo sapiens (Human)
三葉因子3(TFF3)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Trefoil Factor 3, Intestinal (TFF3)48T/96TY11132Mus musculus (Mouse)
低密度脂蛋白(LDL)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Low Density Lipoprotein (LDL)48T/96TY11133Mus musculus (Mouse)
高密度脂蛋白(HDL)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for High Density Lipoprotein (HDL)48T/96TY11134Mus musculus (Mouse)
各種類型ELISA測定時,一般需經過以下步驟:固相包被→加待測樣品→溫育→洗滌+加酶標物→溫育→洗滌→加底物→溫育一加終止液→比色→判定結果。