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小鼠乳酸脫氫酶酶免試劑盒,(LDH)ELISA檢測試劑盒 ,英文名:LDH ELISA Kit ,英文縮寫:LDH ,常見試劑盒規(guī)格:96T/48T,產(chǎn)品別名:小鼠乳酸脫氫酶ELISA檢測試劑盒,LDH 檢測試劑盒, LDH 檢測試劑盒
ELISA實驗操作較繁雜,操作不當(dāng)將引起較大的誤差。小鼠乳酸脫氫酶酶免試劑盒,(LDH)ELISA檢測試劑盒在操作中應(yīng)注意以下5個方面。
1.隨著病情的進展或由其他因素影響,某些抗體在機體內(nèi)的含量發(fā)生大幅波動,因此有必要對標(biāo)本進行預(yù)處理,例如對血清標(biāo)本作適當(dāng)稀釋,或離心分離血脂等。間接法測定中,標(biāo)本適當(dāng)稀釋還可降低非特異性反應(yīng)。
2.加樣一般使用加液器,在ELISA中一般有4次加樣:加標(biāo)本、加酶結(jié)合物、加底物和加終止液。加標(biāo)本時必須每份標(biāo)本使用一支移液器吸嘴,以免交叉污染。加酶結(jié)合物、底物和終止液時則不需更換吸嘴。
3.在成批手工檢測中,還應(yīng)注意操作時差的影響。加樣及混勻時間的差異,使抗原抗體反應(yīng)和酶促反應(yīng)時間不*,對競爭法及定量檢測影響很大,不注意可能會導(dǎo)致錯誤結(jié)果或定量不準(zhǔn)。
4.洗滌是ELISA操作過程中決定實驗成敗的一個關(guān)鍵步驟。目的是除去未結(jié)合的免疫反應(yīng)物,終止抗原抗體反應(yīng),除去標(biāo)本中與反應(yīng)無關(guān)的成分、游離的酶標(biāo)物以及反應(yīng)過程中吸附于固相載體上的非特異性物質(zhì)。可用洗板機洗板或手工洗板,前者洗滌質(zhì)量較好,各反應(yīng)孔洗滌效果均一,批內(nèi)、批間差異小,手工洗板應(yīng)注意控制各孔洗滌效果,差異不宜太大。
5.準(zhǔn)確判定結(jié)果須使用ELISA測讀儀(酶標(biāo)儀),比色法判讀可選擇單波長或雙波長,根據(jù)反應(yīng)液顏色的不同選用不同波長的濾光片,以空白孔校零測定反應(yīng)孔的吸光度值(A值)。酶標(biāo)儀具有良好的重復(fù)性。
轉(zhuǎn)鐵蛋白受體2(TFR2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Transferrin Receptor 2 (TFR2)48T/96TY11785Rattus norvegicus (Rat)
白介素6(IL6)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Interleukin 6 (IL6)48T/96TY11786Bos taurus; Bovine (Cattle)
白介素6(IL6)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Interleukin 6 (IL6)48T/96TY11787Capra hircus; Caprine (Goat)
白介素6(IL6)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Interleukin 6 (IL6)48T/96TY11788Equus caballus; Equine (Horse)
抗腦組織抗體(ABAb)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Anti-Brain Tissue Antibody (ABAb)48T/96TY11789Oryctolagus cuniculus (Rabbit)
白介素8(IL8)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Interleukin 8 (IL8)48T/96TY11790Bos taurus; Bovine (Cattle)
白介素8(IL8)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Interleukin 8 (IL8)48T/96TY11791Capra hircus; Caprine (Goat)
白介素8(IL8)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Interleukin 8 (IL8)48T/96TY11792Equus caballus; Equine (Horse)
白介素9(IL9)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Interleukin 9 (IL9)48T/96TY11793Rhesus monkey (Simian)
抗繆勒管激素(AMH)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Anti Mullerian Hormone (AMH)48T/96TY11794Chicken (Gallus)
抗繆勒管激素(AMH)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Anti Mullerian Hormone (AMH)48T/96TY11795Mus musculus (Mouse)
瘦素受體(LEPR)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Leptin Receptor (LEPR)48T/96TY11796Sus scrofa; Porcine (Pig)
白血病抑制因子(LIF)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Leukemia Inhibitory Factor (LIF)48T/96TY11797Bos taurus; Bovine (Cattle)
白血病抑制因子(LIF)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Leukemia Inhibitory Factor (LIF)48T/96TY11798Canis familiaris; Canine (Dog)
白血病抑制因子(LIF)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Leukemia Inhibitory Factor (LIF)48T/96TY11799Capra hircus; Caprine (Goat)
白血病抑制因子(LIF)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Leukemia Inhibitory Factor (LIF)48T/96TY11800Sus scrofa; Porcine (Pig)
瘦素(LEP)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Leptin (LEP)48T/96TY11801Canis familiaris; Canine (Dog)
瘦素(LEP)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Leptin (LEP)48T/96TY11802Capra hircus; Caprine (Goat)
瘦素(LEP)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Leptin (LEP)48T/96TY11803Equus caballus; Equine (Horse)
L選擇素(SELL)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Selectin, Leukocyte (SELL)48T/96TY11804Bos taurus; Bovine (Cattle)
單核細胞趨化蛋白2(MCP2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Monocyte Chemotactic Protein 2 (MCP2)48T/96TY11805Bos taurus; Bovine (Cattle)
單核細胞趨化蛋白2(MCP2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Monocyte Chemotactic Protein 2 (MCP2)48T/96TY11806Canis familiaris; Canine (Dog)
單核細胞趨化蛋白2(MCP2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Monocyte Chemotactic Protein 2 (MCP2)48T/96TY11807Equus caballus; Equine (Horse)
單核細胞趨化蛋白2(MCP2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Monocyte Chemotactic Protein 2 (MCP2)48T/96TY11808Sus scrofa; Porcine (Pig)
巨噬細胞來源趨化因子(MDC)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Macrophage Derived Chemokine (MDC)48T/96TY11809Bos taurus; Bovine (Cattle)
抗平滑肌抗體(ASMA)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Anti-Smooth Muscle Antibody (ASMA)48T/96TY11810Homo sapiens (Human)
組織蛋白酶K(CTSK)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Cathepsin K (CTSK)48T/96TY11811Rhesus monkey (Simian)
組織蛋白酶K(CTSK)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Cathepsin K (CTSK)48T/96TY11812Bos taurus; Bovine (Cattle)
組織蛋白酶K(CTSK)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Cathepsin K (CTSK)48T/96TY11813Canis familiaris; Canine (Dog)
組織蛋白酶K(CTSK)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Cathepsin K (CTSK)48T/96TY11814Chicken (Gallus)
組織蛋白酶K(CTSK)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Cathepsin K (CTSK)48T/96TY11815Sus scrofa; Porcine (Pig)
組織蛋白酶K(CTSK)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Cathepsin K (CTSK)48T/96TY11816Oryctolagus cuniculus (Rabbit)
胰島素樣生長因子結(jié)合蛋白6(IGFBP6)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Insulin Like Growth Factor Binding Protein 6 (IGFBP6)48T/96TY11817Bos taurus; Bovine (Cattle)
胰島素樣生長因子結(jié)合蛋白6(IGFBP6)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Insulin Like Growth Factor Binding Protein 6 (IGFBP6)48T/96TY11818Capra hircus; Caprine (Goat)
胰島素樣生長因子結(jié)合蛋白6(IGFBP6)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Insulin Like Growth Factor Binding Protein 6 (IGFBP6)48T/96TY11819Equus caballus; Equine (Horse)
胰島素樣生長因子結(jié)合蛋白6(IGFBP6)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Insulin Like Growth Factor Binding Protein 6 (IGFBP6)48T/96TY11820Homo sapiens (Human)
胰島素樣生長因子結(jié)合蛋白6(IGFBP6)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Insulin Like Growth Factor Binding Protein 6 (IGFBP6)48T/96TY11821Sus scrofa; Porcine (Pig)
抑制素結(jié)合蛋白(INHBP)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Inhibin Binding Protein (INHBP)48T/96TY11822Homo sapiens (Human)
抑制素結(jié)合蛋白(INHBP)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Inhibin Binding Protein (INHBP)48T/96TY11823Mus musculus (Mouse)
T-細胞激活連接蛋白(LAT)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Linker For Activation Of T-Cell (LAT)48T/96TY11824Rattus norvegicus (Rat)
轉(zhuǎn)運蛋白1(TNPO1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Transportin 1 (TNPO1)48T/96TY11825Bos taurus; Bovine (Cattle)
轉(zhuǎn)運蛋白1(TNPO1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Transportin 1 (TNPO1)48T/96TY11826Homo sapiens (Human)
巨噬細胞炎性蛋白1α(MIP1α)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Macrophage Inflammatory Protein 1 Alpha (MIP1a)48T/96TY11827Bos taurus; Bovine (Cattle)
巨噬細胞炎性蛋白1α(MIP1α)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Macrophage Inflammatory Protein 1 Alpha (MIP1a)48T/96TY11828Canis familiaris; Canine (Dog)
尿皮質(zhì)素1(UCN1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Urocortin 1 (UCN1)48T/96TY11829Capra hircus; Caprine (Goat)
尿皮質(zhì)素1(UCN1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Urocortin 1 (UCN1)48T/96TY11830Homo sapiens (Human)
尿皮質(zhì)素1(UCN1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Urocortin 1 (UCN1)48T/96TY11831Mus musculus (Mouse)
尿皮質(zhì)素1(UCN1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Urocortin 1 (UCN1)48T/96TY11832Rattus norvegicus (Rat)
巨噬細胞炎性蛋白1β(MIP1β)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Macrophage Inflammatory Protein 1 Beta (MIP1b)48T/96TY11833Bos taurus; Bovine (Cattle)
巨噬細胞炎性蛋白1β(MIP1β)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Macrophage Inflammatory Protein 1 Beta (MIP1b)48T/96TY11834Canis familiaris; Canine (Dog)
巨噬細胞炎性蛋白1β(MIP1β)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Macrophage Inflammatory Protein 1 Beta (MIP1b)48T/96TY11835Chicken (Gallus)
巨噬細胞炎性蛋白1β(MIP1β)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Macrophage Inflammatory Protein 1 Beta (MIP1b)48T/96TY11836Sus scrofa; Porcine (Pig)
巨噬細胞炎性蛋白1β(MIP1β)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Macrophage Inflammatory Protein 1 Beta (MIP1b)48T/96TY11837Oryctolagus cuniculus (Rabbit)
巨噬細胞炎性蛋白1β(MIP1β)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Macrophage Inflammatory Protein 1 Beta (MIP1b)48T/96TY11838Rhesus monkey (Simian)
巨噬細胞炎性蛋白3α(MIP3α)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Macrophage Inflammatory Protein 3 Alpha (MIP3a)48T/96TY11839Bos taurus; Bovine (Cattle)
巨噬細胞炎性蛋白3α(MIP3α)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Macrophage Inflammatory Protein 3 Alpha (MIP3a)48T/96TY11840Canis familiaris; Canine (Dog)
巨噬細胞炎性蛋白3α(MIP3α)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Macrophage Inflammatory Protein 3 Alpha (MIP3a)48T/96TY11841Sus scrofa; Porcine (Pig)
巨噬細胞炎性蛋白3β(MIP3β)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Macrophage Inflammatory Protein 3 Beta (MIP3b)48T/96TY11842Bos taurus; Bovine (Cattle)
巨噬細胞炎性蛋白3β(MIP3β)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Macrophage Inflammatory Protein 3 Beta (MIP3b)48T/96TY11843Canis familiaris; Canine (Dog)
巨噬細胞炎性蛋白3β(MIP3β)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Macrophage Inflammatory Protein 3 Beta (MIP3b)48T/96TY11844Sus scrofa; Porcine (Pig)
各種類型ELISA測定時,一般需經(jīng)過以下步驟:固相包被→加待測樣品→溫育→洗滌+加酶標(biāo)物→溫育→洗滌→加底物→溫育一加終止液→比色→判定結(jié)果。