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小鼠磷脂酰絲氨酸酶免試劑盒,(PS)ELISA檢測試劑盒 ,英文名:PS ELISA Kit ,英文縮寫:PS ,常見試劑盒規(guī)格:96T/48T,產(chǎn)品別名:小鼠磷脂酰絲氨酸ELISA檢測試劑盒,PS 檢測試劑盒, PS 檢測試劑盒
ELISA實驗操作較繁雜,操作不當將引起較大的誤差。小鼠磷脂酰絲氨酸酶免試劑盒,(PS)ELISA檢測試劑盒在操作中應(yīng)注意以下5個方面。
1.隨著病情的進展或由其他因素影響,某些抗體在機體內(nèi)的含量發(fā)生大幅波動,因此有必要對標本進行預(yù)處理,例如對血清標本作適當稀釋,或離心分離血脂等。間接法測定中,標本適當稀釋還可降低非特異性反應(yīng)。
2.加樣一般使用加液器,在ELISA中一般有4次加樣:加標本、加酶結(jié)合物、加底物和加終止液。加標本時必須每份標本使用一支移液器吸嘴,以免交叉污染。加酶結(jié)合物、底物和終止液時則不需更換吸嘴。
3.在成批手工檢測中,還應(yīng)注意操作時差的影響。加樣及混勻時間的差異,使抗原抗體反應(yīng)和酶促反應(yīng)時間不*,對競爭法及定量檢測影響很大,不注意可能會導(dǎo)致錯誤結(jié)果或定量不準。
4.洗滌是ELISA操作過程中決定實驗成敗的一個關(guān)鍵步驟。目的是除去未結(jié)合的免疫反應(yīng)物,終止抗原抗體反應(yīng),除去標本中與反應(yīng)無關(guān)的成分、游離的酶標物以及反應(yīng)過程中吸附于固相載體上的非特異性物質(zhì)。可用洗板機洗板或手工洗板,前者洗滌質(zhì)量較好,各反應(yīng)孔洗滌效果均一,批內(nèi)、批間差異小,手工洗板應(yīng)注意控制各孔洗滌效果,差異不宜太大。
5.準確判定結(jié)果須使用ELISA測讀儀(酶標儀),比色法判讀可選擇單波長或雙波長,根據(jù)反應(yīng)液顏色的不同選用不同波長的濾光片,以空白孔校零測定反應(yīng)孔的吸光度值(A值)。酶標儀具有良好的重復(fù)性。
激肽釋放酶2(KLK2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Kallikrein 2 (KLK2)48T/96TY12235Homo sapiens (Human)
抗神經(jīng)節(jié)苷脂M1抗體(Anti-GM1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Anti-Ganglioside M1 Antibody (Anti-GM1)48T/96TY12236Sus scrofa; Porcine (Pig)
血清淀粉樣P物質(zhì)(SAP)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Serum Amyloid P Component (SAP)48T/96TY12237Mus musculus (Mouse)
谷胱甘肽S轉(zhuǎn)移酶μ2(GSTμ2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Glutathione S Transferase Mu 2, Muscle (GSTm2)48T/96TY12238Homo sapiens (Human)
谷胱甘肽S轉(zhuǎn)移酶α4(GSTα4)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Glutathione S Transferase Alpha 4 (GSTa4)48T/96TY12239Homo sapiens (Human)
成肌分化蛋白(MyoD)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Myogenic Differentiation (MyoD)48T/96TY12240Rattus norvegicus (Rat)
Smad同源物3(Smad3)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Mothers Against Decapentaplegic Homolog 3 (Smad3)48T/96TY12241Homo sapiens (Human)
膜聯(lián)蛋白A11(ANXA11)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Annexin A11 (ANXA11)48T/96TY12242Homo sapiens (Human)
膜聯(lián)蛋白A1(ANXA1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Annexin A1 (ANXA1)48T/96TY12243Homo sapiens (Human)
過氧化還原酶5(PRDX5)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Peroxiredoxin 5 (PRDX5)48T/96TY12244Homo sapiens (Human)
氧化低密度脂蛋白(OxLDL)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Oxidized Low Density Lipoprotein (OxLDL)48T/96TY12245Oryctolagus cuniculus (Rabbit)
橋粒斑蛋白(DSP)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Desmoplakin (DSP)48T/96TY12246Homo sapiens (Human)
血管內(nèi)皮生長因子165(VEGF165)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Vascular Endothelial Growth Factor 165 (VEGF165)48T/96TY12247Bos taurus; Bovine (Cattle)
促卵泡素(FSH)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Follicle Stimulating Hormone (FSH)48T/96TY12248Mus musculus (Mouse)
胃動蛋白1(GKN1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Gastrokine 1 (GKN1)48T/96TY12249Homo sapiens (Human)
α2-巨球蛋白(α2M)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Alpha-2-Macroglobulin (a2M)48T/96TY12250Rattus norvegicus (Rat)
纖維膠凝蛋白1(FCN1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Ficolin 1 (FCN1)48T/96TY12251Homo sapiens (Human)
甘露聚糖結(jié)合凝集素關(guān)聯(lián)絲氨酸蛋白酶2(MASP2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Mannose Associated Serine Protease 2 (MASP2)48T/96TY12252Homo sapiens (Human)
精氨酸(Arg)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Arginine (Arg)48T/96TY12253General
末端補體復(fù)合體C5b-9(C5b-9)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Terminal Complement Complex C5b-9 (C5b-9)48T/96TY12254Homo sapiens (Human)
腫瘤壞死因子相關(guān)凋亡誘導(dǎo)配體(TRAIL)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Tumor Necrosis Factor Related Apoptosis Inducing Ligand (TRAIL)48T/96TY12255Canis familiaris; Canine (Dog)
腫瘤壞死因子相關(guān)凋亡誘導(dǎo)配體(TRAIL)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Tumor Necrosis Factor Related Apoptosis Inducing Ligand (TRAIL)48T/96TY12256Rattus norvegicus (Rat)
簇集蛋白(CLU)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Clusterin (CLU)48T/96TY12257Canis familiaris; Canine (Dog)
胰多肽(PP)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Pancreatic Polypeptide (PP)48T/96TY12258Mus musculus (Mouse)
穿孔素1(PRF1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Perforin 1 (PRF1)48T/96TY12259Rattus norvegicus (Rat)
α1-抗胰蛋白酶(α1AT)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Alpha-1-Antitrypsin (a1AT)48T/96TY12260Rattus norvegicus (Rat)
干擾素γ誘導(dǎo)單核因子(MIγ)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Monokine Induced By Interferon Gamma (MIg)48T/96TY12261Rattus norvegicus (Rat)
干擾素誘導(dǎo)T-細胞α亞族趨化劑(ITαC)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Interferon Inducible T-Cell Alpha Chemoattractant (ITaC)48T/96TY12262Rattus norvegicus (Rat)
緊密連接蛋白1(TJP1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Tight Junction Protein 1 (TJP1)48T/96TY12263Homo sapiens (Human)
誘導(dǎo)型一氧化氮合酶(NOS2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Nitric Oxide Synthase 2, Inducible (NOS2)48T/96TY12264Canis familiaris; Canine (Dog)
層粘連蛋白(LN)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Laminin (LN)48T/96TY12265Homo sapiens (Human)
Endoglin蛋白(ENG)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Endoglin (ENG)48T/96TY12266Rattus norvegicus (Rat)
雄激素受體(AR)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Androgen Receptor (AR)48T/96TY12267Rattus norvegicus (Rat)
阿黑皮素原(POMC)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Proopiomelanocortin (POMC)48T/96TY12268Mus musculus (Mouse)
谷胱甘肽還原酶(GR)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Glutathione Reductase (GR)48T/96TY12269Rattus norvegicus (Rat)
內(nèi)皮素受體A(ETRA)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Endothelin Receptor A (ETRA)48T/96TY12270Homo sapiens (Human)
妊娠相關(guān)血漿蛋白A2(PAPPA2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Pappalysin 2 (PAPPA2)48T/96TY12271Homo sapiens (Human)
補體成分5a(C5a)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Complement Component 5a (C5a)48T/96TY12272Oryctolagus cuniculus (Rabbit)
乳鐵傳遞蛋白(LTF)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Lactoferrin (LTF)48T/96TY12273Equus caballus; Equine (Horse)
乳鐵傳遞蛋白(LTF)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Lactoferrin (LTF)48T/96TY12274Canis familiaris; Canine (Dog)
乳鐵傳遞蛋白(LTF)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Lactoferrin (LTF)48T/96TY12275Capra hircus; Caprine (Goat)
髓過氧化物酶(MPO)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Myeloperoxidase (MPO)48T/96TY12276Bos taurus; Bovine (Cattle)
髓過氧化物酶(MPO)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Myeloperoxidase (MPO)48T/96TY12277Equus caballus; Equine (Horse)
蛋白C抑制因子(PCI)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Protein C Inhibitor (PCI)48T/96TY12278Homo sapiens (Human)
脯氨酰羧肽酶(PRCP)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Prolylcarboxypeptidase (PRCP)48T/96TY12279Rattus norvegicus (Rat)
衰老關(guān)鍵蛋白5(FBLN5)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Fibulin 5 (FBLN5)48T/96TY12280Homo sapiens (Human)
纖維蛋白原(FG)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Fibrinogen (FG)48T/96TY12281Bos taurus; Bovine (Cattle)
牙本質(zhì)涎磷蛋白(DSPP)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Dentin Sialophosphoprotein (DSPP)48T/96TY12282Homo sapiens (Human)
神經(jīng)元特異性烯醇化酶(NSE)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Enolase, Neuron Specific (NSE)48T/96TY12283Oryctolagus cuniculus (Rabbit)
皮質(zhì)醇結(jié)合球蛋白(CBG)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Corticosteroid Binding Globulin (CBG)48T/96TY12284Homo sapiens (Human)
各種類型ELISA測定時,一般需經(jīng)過以下步驟:固相包被→加待測樣品→溫育→洗滌+加酶標物→溫育→洗滌→加底物→溫育一加終止液→比色→判定結(jié)果。