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小鼠孕酮受體酶免試劑盒,(PROGR)ELISA檢測試劑盒 ,英文名:PROGR ELISA Kit ,英文縮寫:PROGR ,常見試劑盒規格:96T/48T,產品別名:小鼠孕酮受體ELISA檢測試劑盒,PROGR 檢測試劑盒, PROGR 檢測試劑盒
ELISA實驗操作較繁雜,操作不當將引起較大的誤差。小鼠孕酮受體酶免試劑盒,(PROGR)ELISA檢測試劑盒 在操作中應注意以下5個方面。
1.隨著病情的進展或由其他因素影響,某些抗體在機體內的含量發生大幅波動,因此有必要對標本進行預處理,例如對血清標本作適當稀釋,或離心分離血脂等。間接法測定中,標本適當稀釋還可降低非特異性反應。
2.加樣一般使用加液器,在ELISA中一般有4次加樣:加標本、加酶結合物、加底物和加終止液。加標本時必須每份標本使用一支移液器吸嘴,以免交叉污染。加酶結合物、底物和終止液時則不需更換吸嘴。
3.在成批手工檢測中,還應注意操作時差的影響。加樣及混勻時間的差異,使抗原抗體反應和酶促反應時間不*,對競爭法及定量檢測影響很大,不注意可能會導致錯誤結果或定量不準。
4.洗滌是ELISA操作過程中決定實驗成敗的一個關鍵步驟。目的是除去未結合的免疫反應物,終止抗原抗體反應,除去標本中與反應無關的成分、游離的酶標物以及反應過程中吸附于固相載體上的非特異性物質??捎孟窗鍣C洗板或手工洗板,前者洗滌質量較好,各反應孔洗滌效果均一,批內、批間差異小,手工洗板應注意控制各孔洗滌效果,差異不宜太大。
5.準確判定結果須使用ELISA測讀儀(酶標儀),比色法判讀可選擇單波長或雙波長,根據反應液顏色的不同選用不同波長的濾光片,以空白孔校零測定反應孔的吸光度值(A值)。酶標儀具有良好的重復性。
解整合素金屬蛋白酶33(ADAM33)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for A Disintegrin And Metalloprotease 33 (ADAM33)48T/96TY13335Homo sapiens (Human)
N-乙酰轉移酶1(NAT1)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for N-Acetyltransferase 1 (NAT1)48T/96TY13336Mus musculus (Mouse)
氯離子通道輔助蛋白1(CLCA1)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Chloride Channel Accessory 1 (CLCA1)48T/96TY13337Homo sapiens (Human)
絲氨酸肽酶抑制因子Kunitz型2(SPINT2)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Serine Peptidase Inhibitor Kunitz Type 2 (SPINT2)48T/96TY13338Homo sapiens (Human)
NADP依賴性蘋果酶2(ME2)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Malic Enzyme 2, NADP+ Dependent, Mitochondrial (ME2)48T/96TY13339Homo sapiens (Human)
干擾素γ誘導單核因子(MIγ)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Monokine Induced By Interferon Gamma (MIg)48T/96TY13340Mus musculus (Mouse)
賴氨酰氧化酶樣蛋白2(LOXL2)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Lysyl Oxidase Like Protein 2 (LOXL2)48T/96TY13341Mus musculus (Mouse)
原鈣黏素γA2(PCDHγA2)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Protocadherin Gamma A2 (PCDHgA2)48T/96TY13342Homo sapiens (Human)
賴氨酰氧化酶樣蛋白1(LOXL1)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Lysyl Oxidase Like Protein 1 (LOXL1)48T/96TY13343Mus musculus (Mouse)
凝血因子Ⅶ(F7)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Coagulation Factor VII (F7)48T/96TY13344Rattus norvegicus (Rat)
心臟肌球蛋白結合蛋白C(MYBPC3)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Myosin Binding Protein C, Cardiac (MYBPC3)48T/96TY13345Homo sapiens (Human)
UL16結合蛋白2(ULBP2)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for UL16 Binding Brotein 2 (ULBP2)48T/96TY13346Homo sapiens (Human)
信號素4A(SEMA4A)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Semaphorin 4A (SEMA4A)48T/96TY13347Homo sapiens (Human)
Tolloid樣蛋白1(TLL1)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Tolloid Like Protein 1 (TLL1)48T/96TY13348Homo sapiens (Human)
絡絲蛋白(RL)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Reelin (RL)48T/96TY13349Mus musculus (Mouse)
精氨酸酶(Arg)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Arginase (Arg)48T/96TY13350Rattus norvegicus (Rat)
含RNA結合冷休克域蛋白E1(CSDE1)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Cold Shock Domain Containing Protein E1, RNA Binding (CSDE1)48T/96TY13351Homo sapiens (Human)
C-型凝集素域家族11成員A(CLEC11A)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for C-Type Lectin Domain Family 11, Member A (CLEC11A)48T/96TY13352Homo sapiens (Human)
絲氨酸蛋白酶23(PRSS23)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Protease, Serine 23 (PRSS23)48T/96TY13353Homo sapiens (Human)
巢蛋白2(NID2)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Nidogen 2 (NID2)48T/96TY13354Homo sapiens (Human)
巢蛋白2(NID2)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Nidogen 2 (NID2)48T/96TY13355Rattus norvegicus (Rat)
FlightlessⅠ同源物(FLIⅠ)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Flightless I Homolog (FLII)48T/96TY13356Homo sapiens (Human)
Thy1細胞表面抗原(Thy1)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Thy1 Cell Surface Antigen (Thy1)48T/96TY13357Rattus norvegicus (Rat)
吲哚胺2,3-雙加氧酶(IDO)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Indoleamine-2,3-Dioxygenase (IDO)48T/96TY13358Mus musculus (Mouse)
沉默調節蛋白1(SIRT1)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Sirtuin 1 (SIRT1)48T/96TY13359Mus musculus (Mouse)
肥大細胞類糜蛋白酶1(CMA1)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Chymase 1, Mast Cell (CMA1)48T/96TY13360Rattus norvegicus (Rat)
促泌素(SCGN)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Secretagogin (SCGN)48T/96TY13361Homo sapiens (Human)
多巴脫羧酶(DDC)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Dopa Decarboxylase (DDC)48T/96TY13362Homo sapiens (Human)
干擾素α4(IFNα4)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Interferon Alpha 4 (IFNa4)48T/96TY13363Homo sapiens (Human)
熱休克蛋白β8(HSPβ8)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Heat Shock Protein Beta 8 (HSPb8)48T/96TY13364Mus musculus (Mouse)
運動神經元生存蛋白2(SMN2)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Survival Of Motor Neuron 2, Centromeric (SMN2)48T/96TY13365Homo sapiens (Human)
γ-谷氨?;D氨酶5(γGT5)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Gamma-Glutamyltransferase 5 (gGT5)48T/96TY13366Homo sapiens (Human)
谷氧還蛋白(GLRX)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Glutaredoxin (GLRX)48T/96TY13367Homo sapiens (Human)
解整合素金屬蛋白酶19(ADAM19)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for A Disintegrin And Metalloprotease 19 (ADAM19)48T/96TY13368Homo sapiens (Human)
白細胞免疫球蛋白樣受體亞家族A成員3(LILRA3)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Leukocyte Immunoglobulin Like Receptor Subfamily A, Member 3 (LILRA3)48T/96TY13369Homo sapiens (Human)
胰島素樣蛋白3(INSL3)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Insulin Like Protein 3 (INSL3)48T/96TY13370Rattus norvegicus (Rat)
鈣結合蛋白(CALB)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Calbindin (CALB)48T/96TY13371Rattus norvegicus (Rat)
聯會復合體蛋白3(SYCP3)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Synaptonemal Complex Protein 3 (SYCP3)48T/96TY13372Homo sapiens (Human)
結腸癌轉移關聯基因1(MACC1)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Metastasis Associated In Colon Cancer 1 (MACC1)48T/96TY13373Homo sapiens (Human)
趨化因子C-C-基元受體7(CCR7)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Chemokine C-C-Motif Receptor 7 (CCR7)48T/96TY13374Mus musculus (Mouse)
整合素α3(ITGα3)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Integrin Alpha 3 (ITGa3)48T/96TY13375Mus musculus (Mouse)
外核苷酸焦磷酸酶/磷酸二酯酶1(ENPP1)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Ectonucleotide Pyrophosphatase/Phosphodiesterase 1 (ENPP1)48T/96TY13376Homo sapiens (Human)
復合素2(CPLX2)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Complexin 2 (CPLX2)48T/96TY13377Homo sapiens (Human)
組蛋白脫乙?;?(HDAC2)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Histone Deacetylase 2 (HDAC2)48T/96TY13378Mus musculus (Mouse)
自噬相關16樣蛋白1(ATG16L1)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Autophagy Related Protein 16 Like Protein 1 (ATG16L1)48T/96TY13379Homo sapiens (Human)
腺苷酸環化酶關聯蛋白2(CAP2)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Adenylyl Cyclase Associated Protein 2 (CAP2)48T/96TY13380Homo sapiens (Human)
Rac-GTP酶激活蛋白1(RACGAP1)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Rac-GTPase Activating Protein 1 (RACGAP1)48T/96TY13381Homo sapiens (Human)
BTG3關聯核蛋白(BANP)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for BTG3 Associated Nuclear Protein (BANP)48T/96TY13382Homo sapiens (Human)
重肽鐵蛋白(FTH)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Ferritin, Heavy Polypeptide (FTH)48T/96TY13383Mus musculus (Mouse)
生長關聯蛋白43(GAP43)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Growth Associated Protein 43 (GAP43)48T/96TY13384Mus musculus (Mouse)
各種類型ELISA測定時, 一般需經過以下步驟:固相包被→加待測樣品→溫育→洗滌+加酶標物→溫育→洗滌→加底物→溫育一加終止液→比色→判定結果。